Tried 2 vials, second was added after first showed no signs of activity ~36hours, both had ~20mls of slurry.
No activity in ~72 hours, just stinky oxidized yeast slurry/wort.
Maybe wyeast 3944 is particularly frost sensitive... Maybe there were the purported killing thaw/freeze cycles...I'll try again w/ the 1056 samples, to see if those guys are still viable.
The differences between the sucess w/ the 1056 and failed 3944 (in order of perceived/wild guess of impact)
- Duration of freeze before reanimation attempt
- Strains
- Volume of slurry in 50ml vial.
- Generations
Posts
I'm thinking that there should be a period of time from the placement into the glycerol solution before freezing. My understanding is that the glycerol (glycerin) needs to be inside the yeast cells in order to prevent cell membrane damage.
ReplyDelete